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Research Article
Platelet transfusion: A study of methods of preparation, storage, quality control, and indications of whole blood-derived platelet concentrates
1 College of Applied Medical Sciences, Al-Dawadmi, Shaqra University, Ministry of Higher Education, Kingdom of Saudi Arabia
2 College of Applied Medical Sciences, Shaqra, Shaqra University, Ministry of Higher Education, Kingdom of Saudi Arabia
3 Department of Laboratory Medicine, Aster Mims Hospital, Kozhikode, Kerala, India
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Suhas K Thazha
College of Applied Medical Sciences, Al-Dawadmi, Shaqra University, Ministry of Higher Education,
Kingdom of Saudi Arabia
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Article ID: 100049Z02ST2019
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How to cite this article
Thazha SK, Scaria B, Mohammed RGA, Rengan SS. Platelet transfusion: A study of methods of preparation, storage, quality control, and indications of whole blood-derived platelet concentrates. Int J Blood Transfus Immunohematol 2019;9:100049Z02ST2019.ABSTRACT
Aims: To study the methods of preparation and storage of whole blood inferred platelet concentrates prepared by the platelet abundant plasma technique, assess the quality control (QC) parameters of whole blood inferred platelet concentrates and study the indications of platelet concentrate transfusion.
Methods: The materials for the present study are a prospective study and were obtained from medico-oncological patients who underwent platelet transfusion therapy with the whole blood-derived platelet concentrates processed by platelet abundant plasma technique in the blood bank of a tertiary care hospital at Kozhikode, Kerala, India during the period of February 2018 to July 2018. The study undertaken in three parts: (1) Study of methods of preparation and storage of whole bloodinduced platelet-rich plasma (PRP) method, (2) Assessment of QC parameters of PRP platelet concentrates, and (3) Study of indications of platelet transfusion therapy in a tertiary care hospital for a period of six months.
Results: In this study, 56 units of whole blood were collected and prepared 56 units of platelet concentrate. One percent of the 56 units prepared was tested of which 75% conformed to the platelet count of not less than 3.5 × 1010 and 4.5 × 1010 and 58% of units were found to have a platelet count of more than 4.5 × 1010. Hundred percent of the volume of platelet units in this study were between 40 and 70 mL and the pH value of all the units were >6.2. The white blood cell (WBC) and red blood cell (RBC) contamination of all the units in this study were less than 1.5 × 109/L and 0.1 × 1012/L, respectively. Eighty-six percent of platelet concentrate units in this study were transfused to patients with hematological malignancies and the remaining 19%, 3%, 3% were transfused to nonhematologic malignancies, immune thrombocytopenic purpura, and disseminated infections, respectively.
Conclusion: In conclusion, it was revealed that only minority patients with malignancy required platelet transfusion; nevertheless, platelets were more usually transfused to patients with malignancy than to patients with some other kind of disease.
Keywords: Malignancy, pH, Platelet concentrate units, RBC contamination, WBC contamination, Transfusion
SUPPORTING INFORMATION
Author Contributions
Suhas K Thazha - Conception of the work, Design of the work, Acquisition of data, Analysis of data, Drafting the work, Revising the work critically for important intellectual content, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.
Bibin Scaria - Conception of the work, Design of the work, Acquisition of data, Analysis of data, Drafting the work, Revising the work critically for important intellectual content, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.
Ramieldin GA Mohammed - Conception of the work, Design of the work, Acquisition of data, Analysis of data, Drafting the work, Revising the work critically for important intellectual content, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.
Sameesh S Rengan - Conception of the work, Design of the work, Acquisition of data, Analysis of data, Drafting the work, Revising the work critically for important intellectual content, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.
Guarantor of SubmissionThe corresponding author is the guarantor of submission.
Source of SupportNone
Consent StatementWritten informed consent was obtained from the patient for publication of this article.
Data AvailabilityAll relevant data are within the paper and its Supporting Information files.
Conflict of InterestAuthors declare no conflict of interest.
Copyright© 2019 Suhas K Thazha et al. This article is distributed under the terms of Creative Commons Attribution License which permits unrestricted use, distribution and reproduction in any medium provided the original author(s) and original publisher are properly credited. Please see the copyright policy on the journal website for more information.
