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Research Article
Quality standards and thromboelastography in platelet pools at the Hospital de Especialidades Centro Medico Nacional Siglo XXI: Instituto Mexicano del Seguro Social
1 Hospital de Especialidades, Centro Médico Nacional Siglo XXI, Mexico City, Mexico
2 DAI de México, SA de CV, Mexico City, Mexico
3 Macopharma SA, Tourcoing, France
Address correspondence to:
Ludwig R Frontier Ramos
3075 Breckinridge Blvd, Suite 405, Duluth, GA 30096,
USA
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Article ID: 100087Z02GA2025
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Benítez-Arvizu G, Castillo-Mercado I, Vanegas-Hernández E, Gonzalez-Carreón MG, Vasquez-Valenzo A, Lopez E, Domínguez V, Arriaga E, Díaz A, Frontier-Ramos LR. Quality standards and thromboelastography in platelet pools at the Hospital de Especialidades Centro Medico Nacional Siglo XXI: Instituto Mexicano del Seguro Social. Int J Blood Transfus Immunohematol 2025;15(2):1–8.ABSTRACT
Aims: Faced with uncertainty about why pooling platelet products for an adequate therapeutic dose is uncommon, the National Medical Center Siglo XXI assessed the quality of blood components from whole blood. To determine their effectiveness, platelets (PLTs) suspended in an additive solution were evaluated for functionality using thromboelastography (TEG).
Methods: Six hundred whole blood samples were centrifuged and separated to obtain red blood cells (RBC), plasma (PL), and buffy coat (BC). The RBCs were immediately filtered in-line to obtain pre-storage leukodepleted red blood cell concentrate (LRBC). The BCs, after an overnight hold, were pooled with an additive solution. Subsequently, the BC pools were centrifuged and separated to obtain pre-storage leukodepleted platelet concentrate (LDPC).
Results: Leukodepleted red blood cell concentrate unit volume was 253 ± 17.8 mL, compliance of 99.3%, resulting in hematocrit (HTC) of 54.8 ± 2.84% and hemoglobin values of 44.5 ± 5.69 g/Unit. Plasma units resulted in a volume of 236.25 ± 51.15 with 70.27 ± 6.95 g/L of total proteins and 145.30 ± 79.25% of Factor VIIIc. Leukodepleted platelet concentrate units yield 2.72 ± 0.41 × 1011 with 0.23 ± 0.26 × 106 residual leukocytes, with a volume of 342 ± 24.67 mL, pH 7 ± 0.08, and all TEG maximum amplitude (MA) values resulted between 75 and 80 mm.
Conclusion: The findings confirm the quality and consistency of blood component collection and processing, with low variability and regulatory compliance. Thromboelastography analysis showed high coagulation capacity in pooled PLTs, highlighting the benefits of standardized pooling for transfusion medicine and healthcare sustainability in Latin America.
Keywords: Buffy coat, Leukodepletion, PAS, Platelet, Thromboelastography
SUPPORTING INFORMATION
Acknowledgments
The authors thank Laboratorios DAI de México, SA de CV. for material and logistical support, Macopharma for training and manuscript assistance, and Haemonetics for providing equipment for thromboelastography testing. Special thanks to Dr. Ignacio Alvarez (Macopharma SA) for his guidance in the statistical analysis. In Discussion section, English grammar and syntax were aided by ChatGPT, developed by OpenAI, using the GPT-4-turbo model. However, the authors warrant the factual accuracy, and all of the written and visual content was confirmed free from plagiarism by Trunitin.
Author ContributionsGamaliel Benítez Arvizu - Conception of the work, Design of the work, Analysis of data, Revising the work critically for important intellectual content, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.
Isabel Castillo Mercado - Conception of the work, Design of the work, Analysis of data, Drafting the work, Revising the work critically for important intellectual content, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.
Elizabeth Vanegas Hernández - Conception of the work, Design of the work, Acquisition of data, Analysis of data, Drafting the work, Revising the work critically for important intellectual content, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.
María Guadalupe González Carreón - Acquisition of data, Drafting the work, Revising the work critically for important intellectual content, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.
Alonso Vázquez Valenzo - Acquisition of data, Analysis of data, Revising the work critically for important intellectual content, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.
Edgar Lopez - Conception of the work, Design of the work, Acquisition of data, Analysis of data, Revising the work critically for important intellectual content, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.
Vianney Domínguez - Acquisition of data, Revising the work critically for important intellectual content, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.
Eleonor Arriaga - Conception of the work, Design of the work, Revising the work critically for important intellectual content, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.
Allexandra Díaz - Conception of the work, Design of the work, Acquisition of data, Analysis of data, Drafting the work, Revising the work critically for important intellectual content, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.
Ludwig R Frontier Ramos - Analysis of data, Drafting the work, Revising the work critically for important intellectual content, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.
Guarantor of SubmissionThe corresponding author is the guarantor of submission.
Source of SupportNone
Consent StatementWritten informed consent was obtained from the patient for publication of this article.
Data AvailabilityAll relevant data are within the paper and its Supporting Information files.
Conflict of InterestAuthors declare no conflict of interest.
Copyright© 2025 Gamaliel Benítez Arvizu et al. This article is distributed under the terms of Creative Commons Attribution License which permits unrestricted use, distribution and reproduction in any medium provided the original author(s) and original publisher are properly credited. Please see the copyright policy on the journal website for more information.
