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Short Report
Spectrum of clinically significant anti-M antibody in patients requiring transfusion
1 Department of Transfusion Medicine, Postgraduate Institute of Medical Education and Research (PGIMER), Chandigarh 160012, India
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Ashish Jain
Professor, Department of Transfusion Medicine, Postgraduate Institute of Medical Education and Research (PGIMER), Chandigarh 160012,
India
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Article ID: 100067Z02AJ2022
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Jain A, Singh L, Kaur A, Sharma RR. Spectrum of clinically significant anti-M antibody in patients requiring transfusion. Int J Blood Transfus Immunohematol 2022;12:100067Z02AJ2022.ABSTRACT
Aims: The study aimed to determine the frequency and serological characteristics of anti-M antibody in patient population.
Methods: It was a retrospective study over for a period of two years (April, 2016 to March, 2018). Blood grouping was performed by tube technique. In case of incompatible cross-match, antibody screening and identification were performed using LISS Coombs’ anti-human globulin (AHG) gel cards (Bio-Rad, Switzerland). Di-thiothreitol (DTT) treatment was also performed to demonstrate the existence of a potentially clinical significant IgG antibody.
Results: The frequency of anti-M antibody was 0.05% in patients (51 out of 101,364 requisitions). DTT treatment could be done in 12 samples which showed it was IgM type in 11 (91.7%) and mixture of IgM and IgG type in 1 (8.3%) case. Transfusion history was present in 21 (41.2%), absent in 13 (25.5%), and not known in 17 (33.3%) patients. M antigen phenotyping in 42 patients showed that 41/42 patients were M-negative (six of them gave a “mixed-field” agglutination due to recent transfusion of M+ PRBC unit). The 42nd patient was typed as M+ which could be again due to history of recent transfusion of PRBC.
Conclusion: Most of the anti-M antibodies were IgM type, however, serological characterization revealed the IgG type also which were found to be reactive at 37°C in AHG phase and thus clinically significant.
Keywords: Alloantibody, Anti-M, Clinically significant, M antigen
SUPPORTING INFORMATION
Author Contributions
Ashish Jain - Conception of the work, Design of the work, Acquisition of data, Analysis of data, Drafting the work, Revising the work critically for important intellectual content, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.
Lakhvinder Singh - Analysis of data, Drafting the work, Revising the work critically for important intellectual content, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.
Arunpreet Kaur - Acquisition of data, Analysis of data, Drafting the work, Revising the work critically for important intellectual content, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.
Ratti Ram Sharma - Conception of the work, Design of the work, Analysis of data, Drafting the work, Revising the work critically for important intellectual content, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.
Guarantor of SubmissionThe corresponding author is the guarantor of submission.
Source of SupportNone
Consent StatementWritten informed consent was obtained from the patient for publication of this article.
Data AvailabilityAll relevant data are within the paper and its Supporting Information files.
Conflict of InterestAuthors declare no conflict of interest.
Copyright© 2022 Ashish Jain et al. This article is distributed under the terms of Creative Commons Attribution License which permits unrestricted use, distribution and reproduction in any medium provided the original author(s) and original publisher are properly credited. Please see the copyright policy on the journal website for more information.
