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Research Article
Comparative in vitro evaluation between platelet concentrates from buffy-coat pooling storage in 100% plasma and 80% platelet additive solution SSP+
1 Product Specialist, Macopharma do Brasil, Brazil
2 Senior Medical Manager, Macopharma, Tourcoing, France
3 Medical Department Director, Macopharma, Tourcoing, France
4 Head of Blood Bank, UMAE Hospital de Especialidades, Puebla, Mexico
5 Quality Control Coordinator of Blood Bank, UMAE Hospital de Especialidades, Puebla, Mexico
6 Scientific Advisor, Laboratorios DAI de México, S.A. de C.V, Ciudad de México, Mexico
Address correspondence to:
Dalia Moreno
Calle 45D No. 81-10 apto 1501, Medellín, Antioquia, Code: 050031,
Colombia
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Article ID: 100058Z02DM2021
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How to cite this article
Moreno D, Frontier L, Álvarez I, Ibarra Y, Morales Y, Domínguez CV. Comparative in vitro evaluation between platelet concentrates from buffy-coat pooling storage in 100% plasma and 80% platelet additive solution SSP+. Int J Blood Transfus Immunohematol 2021;11:100058Z02DM2021.ABSTRACT
Aims: To compare the quality of platelet concentrates obtained from buffy-coat stored in pooled plasma during days 1–5, those stored in additive solution during days 1–5 and 7 processed in UMAE HE Puebla Blood Bank, to evaluate the efficacy of platelet additive solutions.
Methods: Leukoreduced platelet concentrates (LDPCs) were prepared from pools of four buffy-coats (BCs) suspended in pooled plasma (PP) (March–June 2018); and platelet additive solution (SSP+, Macopharma) (August–October 2019) as a validation process in UMAE HE Blood Bank, Puebla, Mexico. On days 1, 5 (PP), and the 7th day of storage (SSP+), samples were tested for CD45, CD42b, CD62P, and Annexin V (CyAn ADP-Beckman Coulter); pCO2, pO2, and pH (OPTI CCA-TS2); glucose, lactic dehydrogenase (LDH), and Total Proteins (Unicel DXC800-Beckman Coulter); and platelet count and media platelet volume (MPV) (Swilab Alfa—Boule).
Results: Platelet recoveries from BC pooling did not present significant differences between PP and SSP+ methods (p >0.05). pH, MPV, and pO2 results were not affected by the storage medium during the storage period. Leukocyte concentration (CD45) complied in both groups with European standards. Glucose consumption and apoptosis markers were of lower values with PP platelets, and activation markers (CD62P) were higher with LDPC in PP (p > 0.05).
Conclusion: In vitro analysis of the seven-day storage of LDPC in SSP+ revealed stability in pH with a lower expression of CD62p, among other biochemical markers. Therefore, LDPC in SSP+ is a sustainable product for transfusion with the equivalent quality compared with five-day storage platelets in PP.
Keywords: Buffy-coat, Platelet additive solution, Platelet concentrates, Pooling, Storage
SUPPORTING INFORMATION
Acknowledgments
We would like to thank the people responsible for the blood processing at the UMAE HE Puebla Blood Bank for allowing us to carry out all the activities to make this investigation possible, and of course, the directives of the blood bank for their interest and commitment to carry out both investigations which allow us to do this comparative article.
Author ContributionsDalia Moreno - Conception of the work, Design of the work, Drafting the work, Revising the work critically for important intellectual content, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.
Ludwig Frontier - Conception of the work, Design of the work, Drafting the work, Revising the work critically for important intellectual content, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.
Ignacio Álvarez - Conception of the work, Design of the work, Drafting the work, Revising the work critically for important intellectual content, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.
Yolanda Ibarra - Conception of the work, Design of the work, Drafting the work, Revising the work critically for important intellectual content, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.
Yazmín Morales - Conception of the work, Design of the work, Drafting the work, Revising the work critically for important intellectual content, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.
Claudia Vianey Domínguez - Conception of the work, Design of the work, Drafting the work, Revising the work critically for important intellectual content, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.
Guarantor of SubmissionThe corresponding author is the guarantor of submission.
Source of SupportNone
Consent StatementWritten informed consent was obtained from the patient for publication of this article.
Data AvailabilityAll relevant data are within the paper and its Supporting Information files.
Conflict of InterestAuthors declare no conflict of interest.
Copyright© 2021 Dalia Moreno et al. This article is distributed under the terms of Creative Commons Attribution License which permits unrestricted use, distribution and reproduction in any medium provided the original author(s) and original publisher are properly credited. Please see the copyright policy on the journal website for more information.
