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Research Article
Variations of three single nucleotide polymorphisms in ABCG2 modify Jra expression
1 Japanese Red Cross Tohoku Block Blood Center, Sendai, Miyagi, Japan
2 Japanese Red Cross Miyagi Blood Center, Sendai, Miyagi, Japan
3 Japanese Red Cross Central Blood Institute, Tokyo, Japan
4 Department of Cell Science, Institute of Biomedical Sciences, Fukushima Medical University, Fukushima, Japan
5 Department of Advanced Cancer Immunotherapy, Fukushima Medical University, Fukushima, Japan
Address correspondence to:
Hitoshi Ohto
MD, PhD, Fukushima Medical University School of Medicine, Department of Advanced Cancer Immunotherapy, Fukushima City, Fukushima 960-1295,
Japan
Message to Corresponding Author
Article ID: 100047Z02YO2019
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Ogiyama Y, Ito S, Irino M, Hishinuma T, Asano T, Ooyama M, Shimizu H, Nakagawa K, Minegishi M, Osabe T, Ogasawara K, Wada I, Ohto H. Variations of three single nucleotide polymorphisms in ABCG2 modify Jra expression. Int J Blood Transfus Immunohematol 2019;9:100047Z02YO2019.ABSTRACT
Aim: The present study reports a single nucleotide polymorphism (SNP) of ATP-binding cassette, membrane G2 (ABCG2) gene may influence the expression of Jra antigen on red blood cells (RBCs).
Methods: Genomic DNAs from 474 random donors were examined for SNPs, c.-262C/T, c.376C/T, and c.421C/A by polymerase chain reaction. The amount of Jra antigen of RBCs was assayed by flow cytometry using human monoclonal anti-Jra (HIRO-133). We classified them in three groups: Group I (wild type) with no variance of three loci (c.-262C, c.376C, and c.421C), Group II with SNP of c.376T and/or c.421A but with c.-262C, and Group III with SNP loci c.-262T.
Results: Compared to Group I (n = 185), Group II (n = 263) showed decreased Jra expression on RBCs by 20–75%, whereas SNP c.-262T (Group III) increased Jra expression with more than 50%. The predicted open structure of 5′ untranslated region formed by c.-262T may recruit the initiation complex for translation or increase the rate of ribosomal scanning, thereby enhancing the frequency of protein synthesis.
Conclusion: Among a healthy Japanese population, the SNPs c.376C>T and/or c.421C>A downregulated expression of Jra, whereas SNP c.-262C>T augmented Jra expression.
Keywords: ABCG2, Antigen expression, Genetic variation, Jra antigen
SUPPORTING INFORMATION
Author Contributions
Yoshiko Ogiyama - Conception of the work, Design of the work, Acquisition of data, Drafting the work, Revising the work critically for important intellectual content, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.
Shoichi Ito - Conception of the work, Design of the work, Acquisition of data, Analysis of data, Drafting the work, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.
Michiyo Irino - Acquisition of data, Drafting the work, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.
Tomoko Hishinuma - Acquisition of data, Drafting the work, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.
Tomomi Asano - Acquisition of data, Drafting the work, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.
Masanori Ooyama - Acquisition of data, Drafting the work, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.
Hiroshi Shimizu - Conception of the work, Design of the work, Drafting the work, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.
Kunitoshi Nakagawa - Conception of the work, Design of the work, Drafting the work, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.
Masayoshi Minegishi - Conception of the work, Design of the work, Drafting the work, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.
Takahiro Osabe - Conception of the work, Design of the work, Drafting the work, Revising the work critically for important intellectual content, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.
Kenichi Ogasawara - Conception of the work, Design of the work, Drafting the work, Revising the work critically for important intellectual content, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.
Ikuo Wada - Drafting the work, Revising the work critically for important intellectual content, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.
Hitoshi Ohto - Conception of the work, Design of the work, Drafting the work, Revising the work critically for important intellectual content, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.
Guarantor of SubmissionThe corresponding author is the guarantor of submission.
Source of SupportNone
Consent StatementWritten informed consent was obtained from the patient for publication of this article.
Data AvailabilityAll relevant data are within the paper and its Supporting Information files.
Conflict of InterestAuthors declare no conflict of interest.
Copyright© 2019 Yoshiko Ogiyama et al. This article is distributed under the terms of Creative Commons Attribution License which permits unrestricted use, distribution and reproduction in any medium provided the original author(s) and original publisher are properly credited. Please see the copyright policy on the journal website for more information.
