Research Article


Immunogenicity of red cell alloantigens in the Japanese population

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1 Department of Blood Transfusion and Transplantation Immunology, Fukushima Medical University Hospital, Fukushima, Japan

2 Department of Laboratory Medicine, Yale University School of Medicine, New Haven, CT, USA

Address correspondence to:

Hitoshi Ohto

MD, PhD, Fukushima Medical University, Fukushima City, Fukushima 960-1295,

Japan

Message to Corresponding Author


Article ID: 100053Z02MW2020

doi: 10.5348/100053Z02MW2020RA

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How to cite this article

Watanabe M, Ohto H, Tormey CA, Yasuda H, Takano N, Minakawa K, Ono S, Yamada-Abe M, Suzuki H, Sugawara A, Kawabata K, Nollet KE, Ikeda K. Immunogenicity of red cell alloantigens in the Japanese population. Int J Blood Transfus Immunohematol 2020;10:100053Z02MW2020.

ABSTRACT


Aims: To elucidate transfusion-related blood group antigen immunogenicity among Japanese, a retrospective single center study was done.

Methods: For 49,884 transfusion recipients from late 2006 to early 2018, antibodies were tested with two techniques: saline immediate spin test and polyethylene glycol-enhanced indirect antiglobulin test. From male recipients with an average of 4.2 donor exposures, we calculated the immunogenicity of blood group alloantigens with a mathematical model.

Results: Indirect antiglobulin test detected 638 alloantibodies, 391 (1.53%) among 25,563 women and 247 (1.02%) among 24,321 men. Anti-E (489.1 per 100,000), followed by anti-Lea (238.6 per 100,000) and anti-Fyb (100.2 per 100,000) were most frequently found in the whole. Immunogenicity calculations among male recipients based on 4-donor exposure revealed Jra (67.4 per 1000, 95% CI: 1.7–323.3) as the possibly highest potency antigen, followed by: Lea (23.5 per 1000, 95% CI: 17.4–29.6), Dib (20.1 per 1000, 95% CI: 0.5–109.3), E (11.9 per 1000, 95% CI: 9.4–14.4), and Jka (5.7 per 1000, 95% CI: 1.7–9.6) among Japanese male recipients.

Conclusion: Jra and Diego blood group antigens might be highly immunogenic in the Japanese population, but more recipients would need to be investigated to establish statistical significance.

Keywords: Alloantibody, Alloimmunization, Blood group alloantigen, Immunogenicity

SUPPORTING INFORMATION


Author Contributions

Mao Watanabe - Conception of the work, Design of the work, Acquisition of data, Drafting the work, Revising the work critically for important intellectual content, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.

Hitoshi Ohto - Conception of the work, Design of the work, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.

Christopher A Tormey - Conception of the work, Design of the work, Drafting the work, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.

Hiroyasu Yasuda - Conception of the work, Design of the work, Acquisition of data, Drafting the work, Revising the work critically for important intellectual content, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.

Nozomi Takano - Acquisition of data, Drafting the work, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.

Keiji Minakawa - Acquisition of data, Drafting the work, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.

Satoshi Ono - Acquisition of data, Drafting the work, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.

Maiko Yamada-Abe - Acquisition of data, Drafting the work, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.

Hiroe Suzuki - Acquisition of data, Drafting the work, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.

Akiko Sugawara - Acquisition of data, Drafting the work, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.

Kinuyo Kawabata - Acquisition of data, Drafting the work, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.

Kenneth E Nollet - Drafting the work, Revising the work critically for important intellectual content, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.

Kazuhiko Ikeda - Conception of the work, Design of the work, Drafting the work, Revising the work critically for important intellectual content, Final approval of the version to be published, Agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.

Guarantor of Submission

The corresponding author is the guarantor of submission.

Source of Support

None

Consent Statement

Informed consent was obtained and documented for the collection and analysis of all patient-related data used in this study.

Data Availability

All relevant data are within the paper and its Supporting Information files.

Conflict of Interest

Authors declare no conflict of interest.

Copyright

© 2020 Mao Watanabe et al. This article is distributed under the terms of Creative Commons Attribution License which permits unrestricted use, distribution and reproduction in any medium provided the original author(s) and original publisher are properly credited. Please see the copyright policy on the journal website for more information.