Original Article
 
Hematological cellular alterations in plateletpheresis donors
Hanan Mahmoud Fayed1, Sanaa Shaker Ali2, Eman Salah Eldin3
1Assistant Professor and Charge d’affaires, Clinical and Chemical Pathology Department, Faculty of Medicine-South Valley University, Qena, Egypt
2Assistant Professor, Clinical and Chemical Pathology Department, Faculty of Medicine-South Valley University, Qena, Egypt
3Lecturer, Clinical and Chemical Pathology Department, Faculty of Medicine-Assiut University, Assiut-Egypt

Article ID: 100041Z02HF2018
doi: 10.5348/100041Z02HF2018OA

Corresponding Author:
Hanan Mahmoud Fayed,
Qena Faculty of Medicine
South Valley University 6th km Qena Safaga,
Road, Qena, Egypt 82523

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How to cite this article
Fayed HM, Ali SS, Eldin ES. Hematological cellular alterations in plateletpheresis donors. Int J Blood Transfus Immunohematol 2018;8:100041Z02HF2018.


ABSTRACT

Aims: Blood contact with foreign surfaces in the apheresis systems may activate many cell types that return back to the donor. However, the safety issue regarding post-procedure platelet activation is not well assessed. We aimed to evaluate the alterations in hematological parameters and to explore the formation of platelets leukocytes aggregates (PLAs) and/or complexes in healthy donors who underwent first-time plateletpheresis procedure.

Methods: Blood from 100 healthy donors were assessed by BD FACS Calibur flow cytometer for: a) detection of PLAs using (CD41, CD42b, CD61) antibodies against platelets surface molecules to detect its expression on neutrophils, monocytes, lymphocytes, and b) evaluation of red cell mechanical fragility (RBC-MF).

Results: After donation a significant decrement of donor blood cell counts; the percent (%) reduction in hemoglobin (Hb) 7.9(5.1–9.2) (p = 0.017), hematocrit (Hct) 6(2.72-7.81) (p = 0.043) %, residual red cells (p = 0.016), platelet count (PLT) 22.7(9.5–32) (p = 0.031) together with a significant increase in the MPV (p = 0.001), the absolute neutrophil (p = 0.026) and lymphocyte count (p = 0.041) with an insignificant increase in absolute monocyte (p = 0.103), lymphocyte subsets count, CD4:CD8 ratio, and WBC count. However, there was significant increase in the median platelets complexed with neutrophil, lymphocytes, and monocytes.

Conclusion: All donors had a significant drop in all blood counts; none of them manifested features of thrombocytopenia or anemia. However, an increase in PLAs formation provides an evidence of ongoing platelet activation, a platelet-leucocyte interaction that may induce a pre-thrombotic risk. This result is important to consider as it might have potential therapeutic implications. Nevertheless, more prospective studies are essential to establish guidelines for donor safety.

Keywords: Donor safety, Flow cytometry, Platelet-leukocyte aggregates, Single donor platelet


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Acknowledgements
All the technicians in blood transfusion center at a tertiary care hospital of Assiut University Hospitals for their contribution to this work.
Author Contributions
Hanan Mahmoud Fayed – Substantial contributions to conception and design, Acquisition of data, Analysis and interpretation of data, Drafting the article, Revising it critically for important intellectual content, Final approval of the version to be published
Sanaa Shaker Ali – Substantial contributions to conception and design, Acquisition of data, Analysis and interpretation of data, Drafting the article, Revising it critically for important intellectual content, Final approval of the version to be published
Eman Salah Eldin – Substantial contributions to conception and design, Acquisition of data, Analysis and interpretation of data, Drafting the article, Revising it critically for important intellectual content, Final approval of the version to be published
Guarantor of Submission
The corresponding author is the guarantor of submission.
Source of Support
None
Consent Statement
Written informed consent was obtained from the patient for publication of this study.
Conflict of Interest
Author declares no conflict of interest.
Copyright
© 2018 Hanan Mahmoud Fayed et al. This article is distributed under the terms of Creative Commons Attribution License which permits unrestricted use, distribution and reproduction in any medium provided the original author(s) and original publisher are properly credited. Please see the copyright policy on the journal website for more information.