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International Journal of Blood Transfusion and Immunohematology - IJBTI - Review Articles, Original Articles, Short Communication, Case Series, Case Reports, Letter to Editors
 
     
Original Article
 
Frequencies of human platelet antigens (HPA-1, -2, -3, -4, and -5) among the Moroccan blood donors
Zainab Ouabdelmoumene1,2, Houria EL Housse1,2, Fatima Zarati3, Nadia Nourichafi3, Kamal Bouisk3,Mohamed Benajiba4, Norddine Habti1,2,4
1Laboratory of Biotechnology and Experimental Medicine and Biotechnology, Faculty of Medicine and Pharmacy Casablanca University, Hassan II, Morocco
2Laboratory of Hematology, Genetic and Cell Engineering- Faculty of Medicine and Pharmacy Casablanca – Hassan II University of Casablanca, Morocco
3Regional blood transfusion center of Casablanca, Morocco
4National Blood Transfusion Centre of Rabat, Morocco

Article ID: 100038Z02ZO2018
doi: 10.5348/100038Z02ZO2018OA

Corresponding Author:
Zainab Ouabdelmoumene,
Laboratory of Biotechnology and Experimental Medicine,
Faculty of Medicine and Pharmacy, University Hassan II,
19 Rue Tarik Ibnou Ziad,
B.P. 9154, Casablanca 20000, Morocco

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How to cite this article
Ouabdelmoumene Z, El housse H, Zarati F, Nourichafi N, Bouisk K, Benajiba M, Habti N. Frequencies of human platelet antigens (HPA-1, -2, -3, -4, and -5) among the Moroccan blood donors. Int J Blood Transfus Immunohematol 2018;8:100038Z02ZO2018.


ABSTRACT

Aims: Human platelet antigens (HPA) are involved in several clinical conditions, such as neonatal alloimmune thrombocytopenia (NAIT), platelet transfusion purpura (PTP), and refractoriness to platelet transfusion.The frequency of platelet antigens varies among populations. So far, typing of HPA systems has not been carried on Moroccan population. The frequencies of these antigens, their risk of alloimmunization, and their clinical implications and complications within Moroccan population are unknown. Our purpose is to define allele frequencies and genotypes in Moroccan population of the five HPA-1 to HPA-5 systems. Evaluate of the risk of anti-platelet alloimmunization among Moroccan blood donors, and estimate the mismatch probability of different platelet alloantigens, after random transfusions of platelet concentrates. Methods: The gene polymorphisms of HPA-1, -2, -3, -4, and -5 were determined by the PCRSSP technique on a DNA sample of 110 healthy Moroccan blood donors randomly chosen.

Results: Alleles frequencies for the HPA systems were: HPA-1a: 0.704, HPA-2a: 0.709, HPA3a: 0.773, HPA-4a: 0.99, and HPA-5a: 0.760. The alleles were HPA-1b: 0.296, HPA-2b: 0.291, HPA-3b: 0.227, HPA-4b: 0.01, and HPA-5b: 0.240. The theoretical frequencies of descendants at risk of alloimmunization are ranged between 0.99% for HPA-4 to 20.76% for HPA-1. The estimated mismatch probability regarding platelet antigens HPA-1, -2, -3, -4, and –5 in Moroccan blood donors, after random platelet transfusion, varies from 1.96% for HPA-4, to 32.9% for HPA-1.

Conclusion: Taking consideration of the previous studies, and our results, a clinical research associated with platelet disorders, such as: neonatal alloimmune thrombocytopenia (FNAIT), post transfusion purpura (PTP), and, multi-platelet transfusion refractoriness (MPR), are needed to ensure the proper diagnosis and the blood transfusion safety.

Keywords: Alloimmunization, Blood donors, HPA-polymorphism, Moroccan population, Platelet transfusion


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Acknowledgements
The authors express their thanks to the blood donors of Regional Blood Transfusion Center of Casablanca. Laboratory of Hematology Cell and Genetic Engineering Faculty of Medicine and Pharmacy Casablanca University Hassan II Morocco Team, and to the National Blood Transfusion Centre of Rabat Morocco
Author Contributions
Zainab Ouabdelmoumene – Substantial contributions to conception and design, Acquisition of data, Analysis and interpretation of data, Drafting the article, Revising it critically for important intellectual content, Final approval of the version to be published
Houria EL Housse – Substantial contributions to conception and design, Acquisition of data, Analysis and interpretation of data, Drafting the article, Revising it critically for important intellectual content, Final approval of the version to be published
Fatima Zarati – Substantial contributions to conception and design, Acquisition of data, Analysis and interpretation of data, Drafting the article, Revising it critically for important intellectual content, Final approval of the version to be published
Nadia Nourichafi – Substantial contributions to conception and design, Acquisition of data, Analysis and interpretation of data, Drafting the article, Revising it critically for important intellectual content, Final approval of the version to be published
Kamal Bouisk – Substantial contributions to conception and design, Acquisition of data, Analysis and interpretation of data, Drafting the article, Revising it critically for important intellectual content, Final approval of the version to be published
Mohamed Benajiba – Substantial contributions to conception and design, Acquisition of data, Analysis and interpretation of data, Drafting the article, Revising it critically for important intellectual content, Final approval of the version to be published
Norddine Habti – Substantial contributions to conception and design, Acquisition of data, Analysis and interpretation of data, Drafting the article, Revising it critically for important intellectual content, Final approval of the version to be published
Guarantor of Submission
The corresponding author is the guarantor of submission.
Source of Support
None
Consent Statement
Written informed consent was obtained from the patient for publication of this study.
Conflict of Interest
Author declares no conflict of interest.
Copyright
© 2018 Zainab Ouabdelmoumene et al. This article is distributed under the terms of Creative Commons Attribution License which permits unrestricted use, distribution and reproduction in any medium provided the original author(s) and original publisher are properly credited. Please see the copyright policy on the journal website for more information.



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