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International Journal of Blood Transfusion and Immunohematology - IJBTI - Review Articles, Original Articles, Short Communication, Case Series, Case Reports, Letter to Editors
 
     
Original Article
 
Extensive human platelet specific antigens typing of blood donors of different geographical origin to manage platelet transfusion in alloimmunized patients: Experience from a transfusion center in Northeastern Italy
Donatella Londero1, Mauro Miani1, Cristina Rinaldi1, Vivianna Totis1, Vincenzo de Angelis1
1Immunohematology Laboratory, Department of Transfusion Medicine, Azienda Sanitaria Universitaria Integrata di Udine, Italy

Article ID: 100036IJBTIDL2018
doi: 10.5348/ijbti-2018-36-OA-2

Corresponding Author:
Dr. Donatella Londero,
Immunohematology Laboratory, Department of Transfusion Medicine,
Azienda Sanitaria Universitaria Integrata di Udine,
Via Pozzuolo 330, 33100 Udine (Italy)

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How to cite this article
Londero D, Miani M, Rinaldi C, Totis V, Angelis VD. Extensive human platelet specific antigens typing of blood donors of different geographical origin to manage platelet transfusion in alloimmunized patients: Experience from a transfusion center in Northeastern Italy. Int J Blood Transfus Immunohematol 2018;8:4–11.


Abstract

Aims: As a consequence of global migration flows the risk of HPA alloimmunization may be increased for recipients of platelets from ethnically diverse donors. In this study, the frequencies of HPA in donors of different geographical origin were determined in a Northeastern Italy Transfusion Department.
Methods: One hundred and ninety-nine apheresis platelet donors, including Europeans (n = 154) and North Africans (n = 29), were enrolled. DNA was extracted with a commercial kit (Bee-Robotic). Extensive HPA genotyping was performed with BloodChip ID HPA (Progenika-Grifols). Chi-squared test for Hardy-Weinberg equilibrium was used to compare frequencies.
Results: The allele a was clearly predominant for HPA 1-11 in both European and North African donors. The allele b was absent for HPA 6-7-8-10-11 in Europeans and for HPA 4-6-7-8-9-10-11 in North Africans. For HPA-15, allele b was more frequent than allele a in North Africans. One case of HPA-4ab and one case of HPA-9abw were detected in Europeans. All HPA genotypes were consistent with Hardy-Weinberg equilibrium. Gene frequencies between both ethnic groups were similar excepting HPA-2 (p = 0.0342).
Conclusion: In our population, there should not be clinically relevant increased risk of post-transfusion purpura or platelet transfusion refractoriness secondary to antibodies to HPAs for recipients of platelet concentrates from blood donors of European and North African ethnicity.

Keywords: Apheresis-donation, Genotyping, Platelet antigens, Platelet-transfusion


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Acknowledgements
We acknowledge Dr. Alessandro Moro for revising the statistical analysis. Dr. Jordi Bozzo (Grifols) is acknowledged for expert review of the text and editorial assistance in the preparation of the manuscript.

Author Contributions
Donatella Londero – Substantial contributions to conception and design, Acquisition of data, Analysis and interpretation of data, Drafting the article, Revising it critically for important intellectual content, Final approval of the version to be published
Mauro Miani – Substantial contributions to conception and design, Acquisition of data, Analysis and interpretation of data, Drafting the article, Revising it critically for important intellectual content, Final approval of the version to be published
Cristina Rinaldi – Substantial contributions to conception and design, Acquisition of data, Analysis and interpretation of data, Drafting the article, Revising it critically for important intellectual content, Final approval of the version to be published
Vivianna Totis – Substantial contributions to conception and design, Acquisition of data, Analysis and interpretation of data, Drafting the article, Revising it critically for important intellectual content, Final approval of the version to be published
Vincenzo de Angelis – Substantial contributions to conception and design, Acquisition of data, Analysis and interpretation of data, Drafting the article, Revising it critically for important intellectual content, Final approval of the version to be published
Guarantor of Submission
The corresponding author is the guarantor of submission.
Source of Support
None
Conflict of Interest
Authors declare no conflict of interest.
Copyright
© 2018 Donatella Londero et al. This article is distributed under the terms of Creative Commons Attribution License which permits unrestricted use, distribution and reproduction in any medium provided the original author(s) and original publisher are properly credited. Please see the copyright policy on the journal website for more information.



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