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Original Article
 
Preparation of red cell concentrates in low-income countries: Efficacy of whole blood settling method by simple gravity in Burkina Faso
Salam Sawadogo1,2, Kompingnin Nébié1,2, Eléonore Kafando1, Tieba Millogo3, Siaka Ouattara2, Honorine Dahourou2, Georges Ouédraogo2, Sibiri Nana2, Véronique Deneys4
1Hematology Laboratory, University Ouaga I Professeur Joseph KI-ZERBO, Ouagadougou, Burkina Faso.
2National Blood Transfusion Centre, Ouagadougou, Burkina Faso.
3African Institute of Public Health, Ouagadougou, Burkina Faso.
4Transfusion service, CHU UCL Namur, Catholic University of Louvain, Brussels, Belgium.

Article ID: 100025IJBTISS2016
doi:10.5348/ijbti-2016-25-OA-5


Address correspondence to:
Salam Sawadogo
01 BP 865 Ouagadougou 01
Burkina Faso

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How to cite this article
Sawadogo S, Nébié K, Kafando E, Millogo T, Ouattara S, Dahourou H, Ouédraogo G, Nana S, Deneys V. Preparation of red cell concentrates in low-income countries: Efficacy of whole blood settling method by simple gravity in Burkina Faso. Int J Blood Transfus Immunohematol 2016;6:20–29.


Abstract
Aim: This study aims to describe the characteristics of the red cell concentrates (RCCs) produced by whole blood settling in Burkina Faso.
Methods: We conducted a cross-sectional study in the blood centers of Ouagadougou and Koudougou between 12th February and 15th March 2015. 427 blood units selected at Koudougou and divided into three groups were processed by settling method over three durations (methods I = 36–48 h, II = 48–72 h and III = 72–96 h). Other 139 units selected at Ouagadougou were processed by centrifugation (method IV). The RCCs units' characteristics were described by the mean of their hematocrit, volume, hemoglobin level and content. The residual plasma level, the hematocrit yield and the hematocrit concentration ability (ability of method to raise hematocrit in comparison with whole blood) have been used to assess methods efficacy.
Results: The volume and hemoglobin content of overall units met standard requirements. 2.1%, 14.2% and 27.7% of units respectively issued from methods I, II, III had hematocrit over 50%. The average volume (mL) was 372.88, 357.91, 350.30 and 332.26 respectively for method I to IV (p<0.05). The mean hematocrit (%) was respectively 45.42, 47.41, 48.24 and 62.28 (non-significant difference between methods II and III). The hematocrit concentration ability was respectively 1.14, 1.22, 1.24 and 1.51 and the proportion of residual plasma 0.39, 0.33, 0.30 and 0.09.
Conclusion: The RCCs obtained by settling method did not meet all standards. But we can hypothesize that they would be more effective and safe for patients. However, it is necessary to undertake further studies to verify it.

Keywords: Blood sedimentation, Centrifugation, Gravity


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Author Contributions:
Salam Sawadogo – Substantial contributions to conception and design, Acquisition of data, Analysis and interpretation of data, Drafting the article, Revising it critically for important intellectual content, Final approval of the version to be published
Kompingnin Nébié – Substantial contributions to conception and design, Revising it critically for important intellectual content, Final approval of the version to be published
Eléonore Kafando – Substantial contributions to conception and design, Revising it critically for important intellectual content, Final approval of the version to be published
Tieba Millogo – Substantial contributions to conception and design, Analysis and interpretation of data, Revising it critically for important intellectual content, Final approval of the version to be published
Siaka Ouattara – Substantial contributions to conception and design, Analysis and interpretation of data, Revising it critically for important intellectual content, Final approval of the version to be published
Honorine Dahourou – Substantial contributions to conception and design, Analysis and interpretation of data, Revising it critically for important intellectual content, Final approval of the version to be published
Georges Ouédraogo – Acquisition of data, Revising it critically for important intellectual content, Final approval of the version to be published
Sibiri Nana – Acquisition of data, Revising it critically for important intellectual content, Final approval of the version to be published
Véronique Deneys – Substantial contributions to conception and design, Analysis and interpretation of data, Revising it critically for important intellectual content, Final approval of the version to be published
Guarantor of submission
The corresponding author is the guarantor of submission.
Source of support
None
Conflict of interest
Authors declare no conflict of interest.
Copyright
© 2016 Salam Sawadogo et al. This article is distributed under the terms of Creative Commons Attribution License which permits unrestricted use, distribution and reproduction in any medium provided the original author(s) and original publisher are properly credited. Please see the copyright policy on the journal website for more information.