International Journal of Blood Transfusion and Immunohematology - IJBTI - Review Articles, Original Articles, Short Communication, Case Series, Case Reports, Letter to Editors
 
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Original Article
 
Morphological and functional changes in random donor platelets stored for seven days in platelet additive solution
Tulika Chandra1, Ashish Gupta1, 2, Ashutosh Kumar2, Sheeba Afreen1, 2
1Department of Transfusion Medicine and Blood Bank.
2Department of Pathology, Chhatrapati Shahuji Maharaj Medical University (Earlier King Georg's Medical University), Lucknow, Uttar Pradesh, India.

Article ID: 100005IJBTITC2011
doi:10.5348/ijbti-2011-5-OA-5

Address correspondence to:
Dr. Tulika Chandra
Assistant Prof. Department of Transfusion Medicine and Blood Bank
Chhatrapati Shahuji Maharaj Medical University (Earlier King Georg's Medical University)
Lucknow, Uttar Pradesh
India Pin-226003
Phone: +91-9415755536
Email: tulikachandra@rediffmail.com

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How to cite this article:
Chandra T, Gupta A, Kumar A, Afreen S. Morphological and functional changes in random donor platelets stored for seven days in platelet additive solution. International Journal of Blood Transfusion and Immunohematology 2011;1:20-25.


Abstract
Aims: Platelets are small, anucleated cytoplasmic fragments that play an essential role in blood clotting and wound healing. Platelets are routinely stored in plasma for five days at 22°C. Aim: Our objective was to assess platelet morphology and function in random donor platelet stored for seven days in platelet additive solution.
Methods: The random donor platelet was prepared by PRP method. Platelet concentrates from 50 blood donor were stored in 100% plasma (n=24) and 20:80 (n=24) additive solution. The RDP were placed in incubator and agitator at 22°C.
Results: Out of total 50 samples, 48 samples were analyzed and two were discarded due to the bacterial contamination. On comparing the mean values of platelet count and MPV in both the groups with and without platelet additive solution, no significant difference was observed on day seven of storage period. A significant difference in PDW was observed on day seven (p < 0.001) in plasma. The mean values of LDH and pH showed no significant difference on day seven in both the groups with and without platelet additive solution. A significant difference was observed in the levels of glucose on day seven (p < 0.001) in plasma. In platelet aggregation, a significant decrease was seen in plasma (p < 0.001) on day seven at 22°C.
Conclusions: The platelet morphology and function was better maintained in additive solution on day seven as compared to platelets stored without additive solution.

Key Words: Platelet morphology, Platelet function, Random donor platelets, Platelet additive solution, Day

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Author Contributions:
Tulika Chandra - Substantial contributions to conception and design, Analysis and interpretation of data, Revising it critically for important intellectual content, Final approval of the version to be published
Ashish Gupta - Analysis and interpretation of data, Drafting the article, Revising it critically for important intellectual content, Final approval of the version to be published
Ashutosh Kumar - Substantial contributions to conception and design, Drafting the article, Final approval of the version to be published
Sheeba Afreen - Analysis and interpretation of data, Drafting the article, Revising it critically for important intellectual content, Final approval of the version to be published
Guarantor of submission:
The corresponding author is the guarantor of submission.
Source of support:
Council of Science and technology, Uttar Pradesh, Lucknow
Conflict of interest:
Authors declare no conflict of interest.
Copyright:
© Tulika Chandra et al. 2011; This article is distributed the terms of Creative Commons Attribution License which permits unrestricted use, distribution and reproduction in any means provided the original authors and original publisher are properly credited. (Please see Copyright Policy for more information.)



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